Effect of LPS-activated MD-DCs on NK/LAK activation.
LPS-activated MD-DCs are the most potent APCs for NK/LAK cell activation. Cocultures of day-7 immature MD-DCs (iDCs) with or without LPS or mature MD-DCs (mDCs) with 1 × 106 immunopurified day-7 NK/LAK cells per milliliter were performed at 3 NK/MD-DC ratios (1:1, 5:1, and 10:1). Control cultures included NK/LAK alone with or without IL-2 (1000 IU/mL). At days 3 (panel A) and 7 (panel B), NK/LAK cells from the different conditions were counted and assayed for cytotoxicity at 2 E/T ratios (10:1 and 5:1) against K562 (○), Daudi (■), IGR-NB1 (●), and IGR-NB2 (▪). ELISA assay for IFN-γ (panel C) and IL-12–p70 heterodimer production (panel D) were performed on supernatants of day-3 (gray bars) and day-7 (black bars) cocultures. This figure shows a representative of 2 experiments performed on 1 patient.
