Tumor-derived V_H gene and deducted amino acid sequences from case 1.

V_H sequences from blood (Bl), spleen (Sp), and chest wall (ChestW) are aligned against the germ line gene counterpart (V_3-33). Dashes represent identity with the germline sequence. Replacement amino acid changes observed in more than one clone are starred. CDRs are in bold type and underlined. Amino acid numbering is according to Kabat et al.22