ABCG2 surface protein expression in adult specimens.

Selected patient samples were analyzed by flow cytometry. Frozen samples were thawed and labeled with either anti-ABCG2 5D3 or isotype control antibody. The dot plots represent PE fluorescence on the x-axis and forward scatter on the y-axis. Each sample was gated to analyze only PI-negative (live) cells. The top row represents results of labeling with isotype control antibody; the bottom row represents results from labeling with anti-ABCG2 monoclonal antibody 5D3. The percent positive cells was based on a gate derived from the isotype control. The percentage of cells within the positive gate is shown for each sample.