Impaired intracellular processing of FV mutants.

The wt-FV and the FV mutants in cell lysates at 2 hours' chase (upper panel) and 8 hours' chase (middle panel) were subjected to 4% SDS-PAGE under reducing conditions. It should be noted that the gel of 8 hours' chase was overexposed to make faint bands visible. Lane 1, wt-FV; lane 2, mutant Q; lane 3, mutant Met385Thr; lane 4, mutant His1299Arg; lane 5, mutant Met1736Val; lane 6, mutant Asp2194Gly. The radioactivity of the primary translated peptide (band 1) and the posttranslational intermediates (bands 2 and 3) in each lane was quantitated, and the ratios of the posttranslational intermediates over the primary translated peptide (bands 2 and 3/band 1) were calculated (lower panel). Closed and open histograms indicate the ratios at 2 hours' chase and 8 hours' chase, respectively.