Fig. 2.
Fig. 2. Phenotypic profile ofMLRCD4+ T cells. / Pre-G or post-G CD4+ T cells from the same donor were activated with allogeneic TCD PBMCs for 7 days in MLR at a 1:3 stimulator-responder ratio. The expression of informative activation–differentiation antigens was then evaluated with multicolor flow cytometry. (A) Median values and ranges recorded in 6 independent experiments are shown. *P < .05 compared with pre-GMLRCD4+ T cells. (B) A representative set of experiments is depicted. Markers were set according to the proper isotypic control, and the percentage of CD4+ T cells expressing a given antigen is indicated in the upper right quadrant of each bidimensional cytogram.

Phenotypic profile ofMLRCD4+ T cells.

Pre-G or post-G CD4+ T cells from the same donor were activated with allogeneic TCD PBMCs for 7 days in MLR at a 1:3 stimulator-responder ratio. The expression of informative activation–differentiation antigens was then evaluated with multicolor flow cytometry. (A) Median values and ranges recorded in 6 independent experiments are shown. *P < .05 compared with pre-GMLRCD4+ T cells. (B) A representative set of experiments is depicted. Markers were set according to the proper isotypic control, and the percentage of CD4+ T cells expressing a given antigen is indicated in the upper right quadrant of each bidimensional cytogram.

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