Fig. 4.
Fig. 4. Proliferation ofMLRCD4+ T cells. / Pre-G or post-G CD4+ T cells from the same donor were activated with allogeneic TCD PBMCs for 7 days in MLR at a 1:3 stimulator-responder ratio. Proliferation was determined after 7 days of culture by the addition of BrdUrd for the final 24 hours. Proliferation of pre-G or post-G CD4+ T cells in the absence of stimulator cells never exceeded 5% (data not shown). (A) Median values and ranges recorded in 6 independent experiments are plotted. *P < .05 compared with pre-GMLRCD4+ T cells. (B) A representative experiment is shown. Percentage of cells incorporating BrdUrd has been indicated.

Proliferation ofMLRCD4+ T cells.

Pre-G or post-G CD4+ T cells from the same donor were activated with allogeneic TCD PBMCs for 7 days in MLR at a 1:3 stimulator-responder ratio. Proliferation was determined after 7 days of culture by the addition of BrdUrd for the final 24 hours. Proliferation of pre-G or post-G CD4+ T cells in the absence of stimulator cells never exceeded 5% (data not shown). (A) Median values and ranges recorded in 6 independent experiments are plotted. *P < .05 compared with pre-GMLRCD4+ T cells. (B) A representative experiment is shown. Percentage of cells incorporating BrdUrd has been indicated.

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