Thrombin induces the nuclear translocation of SRF in primary endothelial cells.

HPAECs were plated onto 4-well chamber slides, serum-starved overnight, and incubated in the absence (Control) or presence of 1.5 U/mL thrombin for the times indicated. The cells were fixed in ice-cold (4°C) methanol for 10 minutes, washed with PBS, and subsequently incubated with primary anti-SRF antibody, followed by FITC-conjugated second antibody at room temperature. The nuclei were stained with DAPI. Bar, 10 μm. All panels are shown at the same magnification.