Increased JAM-2 expression on transfection of tEnd.1 endothelial cells.

Cytofluorometric analysis of JAM-2 and JAM-1 expression on endothelial cells with or without transfection of exogenous JAM-2: monoclonal antibodies used for staining were anti–JAM-1 (H202 106.7.4, lower panels), anti–JAM-2 (CRAM-13 H33, upper panels) and are represented by solid histograms. Open profiles represent the negative control obtained using the secondary antibody alone. The left panels show nontransfected cells and the right panels show cells transfected with JAM-2.