Anti–JAM-2 blocking of lymphocyte transmigration.

Transmigration assays were carried out as described for Figure 2, except that in some wells, anti–JAM-2 antibody (CRAM-13 H33) was added to the endothelioma monolayers for 30 minutes prior to the addition of lymphocytes. The antibody was left for the duration of the transmigration assay. Results are expressed as the percentage of cells transmigrated, calculated from the mean of triplicate filters (± SD). The degree of inhibition varied between 35% and 50%, and the data presented in this figure are representative of more than 4 experiments; *P < .05.