JAM-2 antibodies cross-react on human JAM-2.

(A) MDCK cells stably transfected with the indicated cDNA were stained with anti–JAM-2 antibody (solid profile) or an irrelevant antibody (dashed profiles). (B) HUVECs were tested for expression of the 3 JAM family members using 3D8 against JAM-1, (left panel, filled profile), CRAM-18F26 against JAM-2, (middle panel, filled profile), or anti–VE-JAM against JAM-3, (right panel, filled profile). Open profiles represent the negative control obtained using the appropriate secondary antibodies alone.