JAM-2 is expressed on HUVECs in monolayers and on human tonsillar HEVs.

(A) Confluent HUVEC monolayers were stained with CRAM-19 H36 ascites and staining was revealed with antirat FITC. (B) Serial sections of human tonsil were stained with either MECA 79 antibody, recognizing carbohydrate epitopes of PNAd on lymphoid tissue HEVs (left panel) or CRAM-19 H 36 ascites, recognizing JAM-2 (right panel) and revealed with antirat FITC. Using the same settings for the CCD, no staining was detectable if primary antibody was omitted.