Transmigration of human PBLs across monolayers of HUVECs is inhibited by anti–JAM-2 antibody and soluble JAM-2.
HUVECs (8 × 104 cells) were cultured per Transwell culture insert. Three days later, the cultures were washed and 8 × 105 PBLs were added to each filter and incubated for 2 hours in the presence of human SDF-1 added to the lower chamber. Fifteen minutes prior to addition of lymphocytes, the indicated reagents were added: panels A and B, anti–JAM-2 (CRAM-18 F26) or isotype-matched control antibody (IgG2a isotype), 2 representative experiments shown, or panel C, soluble JAM-2 or a control soluble peptide (tag D). The transmigrated cells were collected from the lower chamber and counted by light microscopy. Results are expressed as the percentage of cells transmigrated, calculated from the mean of triplicate filters (± SD). The data presented in this figure are representative of 2 experiments. **P < .01.
