Fig. 2.
RT-PCR analysis of p57KIP2 gene expression and MSP analysis of p57KIP2gene promoter region in various cell lines.
Upper arrow shows 162-bp RT-PCR product ofp57KIP2 gene; lower arrow, 225-bp RT-PCR product of GAPDH gene; U, PCR products amplified with primers specific for unmethylated DNA (MSP-U and MSP-A); M, PCR products amplified with primers specific for methylated DNA (MSP-M and MSP-A).