Fig. 3.
Restoration of p57KIP2gene expression in RAJI and HL60 cells by 5-aza-2′-deoxycytidine (5-aza-2′C), detected by RT-PCR.
Top row of panels shows expression ofp57KIP2 gene; second row of panels, expression of GAPDH; third row of panels, detection of unmethylated status of p57KIP2 gene by MSP method; bottom row of panels, detection of methylated status ofp57KIP2 gene by MSP method. Upper arrow, 162-bp RT-PCR product ofp57KIP2 gene; lower arrow, 225-bp RT-PCR product of GAPDH gene.