Fig. 4.
Methylation status of promoter region ofp57KIP2 gene in CpG islands in 2 samples of reactive lymphadenitis, 1 normal PBMNC sample, and 6 cell lines determined by sequencing after bisulfite modification of genomic DNA.
CpG 1 to CpG 46 correspond to what is depicted in Figure 1. Expression of p57 was detected by RT-PCR, and the percentage of methylation was determined from ratios of methylated cytosine in 10 to 12 sequenced clones.
