Time kinetic experiments on IL-1β– or TNF-α–induced activation of cotransfected HMEC-1.

Relative luciferase activity in unstimulated or stimulated cells at 0 hour (A), 2 hours (B), 3 hours and 30 minutes (C), and 5 hours (D) of activation. HMEC-1 were cotransfected with p-CMV empty vector (■), ΔTRAF2 (▤), ΔTRAF6 (▪), ΔMyD88 (▧) and stimulated by IL-1β (50 U/mL) or TNF-α (10 ng/mL). Data are shown as cpm, mean ± SD of 3 different experiments. *P < .05 versus vector.