Fig. 3.
Fig. 3. Time kinetics of IRAK phosphorylation induced by different agonists. / Medium alone (Ctr), IL-1β (1 U/mL), polyclonal anti–β2-GPI IgG (IgG Pos; 200 μg/mL), NHS IgG (IgG Neg; 200 μg/mL), and LPS (10 ng/mL) were used. To evidence equal loading of the lanes, in control experiments one tenth of each supernatant after IRAK immunoprecipitation was run on an SDS-PAGE gel and blotted against β-actin (lower panel).

Time kinetics of IRAK phosphorylation induced by different agonists.

Medium alone (Ctr), IL-1β (1 U/mL), polyclonal anti–β2-GPI IgG (IgG Pos; 200 μg/mL), NHS IgG (IgG Neg; 200 μg/mL), and LPS (10 ng/mL) were used. To evidence equal loading of the lanes, in control experiments one tenth of each supernatant after IRAK immunoprecipitation was run on an SDS-PAGE gel and blotted against β-actin (lower panel).

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