Ex vivo expansion ability of Fanca-deficient bone marrow cells.

(A) Total bone marrow cells from Fanca^+/+(■) and Fanca^−/−(▪) cells were subjected to ex vivo expansion in the presence of 3 different combinations of growth factors. At weekly intervals, cells were counted and diluted to the input concentration (10⁵ cells/mL). Data represent the total cell number generated by the initial 10⁵ cells. Each point represents the mean ± SEM corresponding to 4 independent experiments. *Statistical significance betweenFanca^+/+ and Fanca^−/−samples at P < .05. (B) Lin⁻Sca-1⁺ cells fromFanca^+/+ (○) and Fanca^−/−(●) were purified and ex vivo expanded with SCF and IL-11. Cytometry histograms of bone marrow cells before and after purification of the Lin⁻Sca-1⁺ population and the ex vivo expansion kinetics following SCF and IL-11 stimulation are shown. The number in the histogram indicates the percentage of cells within the statistics quadrant.