Fig. 6.
Fig. 6. Correction of the mitomycin C sensitivity ofFanca−/− progenitors by retroviral vectors encoding the human FANCA gene. / Purified Lin−Sca-1+ cells fromFanca+/+ and Fanca−/−mice were mock infected or infected with retroviral vectors encoding the FANCA and EGFP genes (LFAPEG vector) and then cultured in the presence of increasing concentrations of MMC. Panels A-C represent the mean ± SEM of data corresponding to 5 independent experiments. Panels D-F show data corresponding to one representative experiment that included an additional group transduced with a vector encoding only the EGFP gene (LPEG vector). In panels C and F, triangles indicate infections with LFPEG; circles, infections with LFAPEG vectors; empty symbols, Lin−Sca-1+ cells obtained fromFanca+/+ mice, and solid symbols, Lin−Sca-1+ cells obtained fromFanca−/− mice.

Correction of the mitomycin C sensitivity ofFanca−/− progenitors by retroviral vectors encoding the human FANCA gene.

Purified Lin−Sca-1+ cells fromFanca+/+ and Fanca−/−mice were mock infected or infected with retroviral vectors encoding the FANCA and EGFP genes (LFAPEG vector) and then cultured in the presence of increasing concentrations of MMC. Panels A-C represent the mean ± SEM of data corresponding to 5 independent experiments. Panels D-F show data corresponding to one representative experiment that included an additional group transduced with a vector encoding only the EGFP gene (LPEG vector). In panels C and F, triangles indicate infections with LFPEG; circles, infections with LFAPEG vectors; empty symbols, Lin−Sca-1+ cells obtained fromFanca+/+ mice, and solid symbols, Lin−Sca-1+ cells obtained fromFanca−/−mice.

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