Aggregation of FcεRI induces the association of 3BP2-SH2 domain with LAT.

Cells transfected with vector alone (pMT3) and cells overexpressing 3BP2-SH2 domain were stimulated with antigen for the indicated times and cell lysates were then immunoprecipitated with anti-HA antibody. The immunoprecipitated proteins were separated by 10% and 14% SDS-PAGE and analyzed by immunoblotting with anti-pTyr, anti-LAT, and anti-HA antibodies.