CD detection by RT-PCR.

(A) Amplification of human CD56 in the prepared dilutions of CD56⁺ cells in SP2/0-Ag14 myeloma mouse cell line. (B) Amplification of human CD161 in the same prepared dilutions. (C) Amplification of human CD34 in the prepared dilutions of CD34⁺ in SP2/0-Ag14 myeloma mouse cell line. (D) Amplification of human CD7 in the prepared dilutions of CD56⁺ cells. (E) Amplification of β-actin to monitor the integrity of the mRNA of the samples A-D. (F) Detection of CD mRNA in UCB/CD14⁺ cells: lane 1, CD56; lane 2, CD161; lane 3, CD34; and lane 4, CD7. Identical results from 4 conducted experiments. (G) Amplification of β-actin of the samples in panel F.