Fig. 6.
![Fig. 6. In vitro phosphorylation of cortactin by PAK. / PAK was immunoprecipitated (IP) from platelets stimulated or not with THR (0.5 UI/mL) as indicated and incubated with 5 μg purified rHu cortactin in the presence of 20 μM MgCl2-ATP and 0.185MBq (5 μCi) γ[32P]-ATP. Phosphocortactin (pCortactin) was revealed by autoradiography. PAK was quantified by anti-PAK1/2 immunoblot (IB). Results are representative of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/13/10.1182_blood.v100.13.4462/4/h82423531006.jpeg?Expires=1736063108&Signature=nuVRZZrjrTF7~aQFZkBLoS-ziaQvx~n6vddphGligzmKtrFUj2TC-EAlMggCSicR6bxLB2lRjNuCWkxfKRT9ZellpfdGJictFgqH26o-Lh00PX4GGVLQl0JOtbLdDg8BAn~cPGX8giAAPLywOEMn1mP4srVdLgBcPnrxpH9NTwXWAr7HfYQoxLnSWW3e0GBHhEUnS5lAFzl6ZVdGTsxdLLpeWyquCQa3QaFfOpccKRzrLYE0qnVmGyhPksMIpFYQtCiYndlpUgjgs3xw1Qrkj78wbJrJewCsXfaBeMiOqAAvQJTY6hFatpVTXuF6eQAE~CzxhBMmZunlgw1T3KKHqg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro phosphorylation of cortactin by PAK.
PAK was immunoprecipitated (IP) from platelets stimulated or not with THR (0.5 UI/mL) as indicated and incubated with 5 μg purified rHu cortactin in the presence of 20 μM MgCl2-ATP and 0.185MBq (5 μCi) γ[32P]-ATP. Phosphocortactin (pCortactin) was revealed by autoradiography. PAK was quantified by anti-PAK1/2 immunoblot (IB). Results are representative of 3 separate experiments.
