Mapping of the cdy locus and protein sequence alignment.

(A) Overlapping contig of YAC clones (blue bars) containing thecdy-linked AFLP markers I22, E5, and E59. The YAC clones 130B7, 198D7, and 159D2 all contain both the E59 AFLP marker and the candidate gene dmt1. The interval containing thedmt1 gene is depicted with a black bar. Proximal and distal refer to position with respect to the centromere of LG11. Note that physical distances are not drawn to scale. (B) Sequence alignment of zebrafish, human, and mouse DMT1 proteins (GenBank accession numbers: zebrafish, AF529267; human, AAC21459; and mouse, AAC24496). The zebrafish, human, and mouse predicted open reading frames are 547, 568, and 568, respectively. Note that because no iron response element (IRE) was found in the 3′ untranslated region (UTR) of our zebrafishdmt1 cDNA, the human and mouse C-terminal alternate splice forms (no IRE in 3′UTR) were used for this sequence alignment. Yellow shading indicates identical amino acids. The lysine (K) at position 264 (*) is mutated to a stop codon in the cdy^te216allele. Identification of a nonsense mutation suggests that thecdy mutant gene is zebrafishdmt1.