Fig. 3.
In situ hybridization staining fordmt1 mRNA.
Dark purple staining indicates expression of dmt1 RNA. (A) Four-somites stage: Posterior-dorsal view of an embryo showing expression in the presumptive blood precursors (arrow). (B) Twenty-somites stage: Lateral view showing expression in immature erythroid cells in the intermediate cell mass (blood island of zebrafish embryo). (C) 24 hpf: High magnification of the tail at 24 hours after fertilization (hpf) showing expression in individual erythroid cells. (D) 48 hpf: View of circulating red blood cells expressing dmt1 RNA (arrow) as they flow over the yolk sac on their way into the heart. (E) Day 5, low magnification: Arrows indicate expression in cells of the embryonic intestine. (F) Day 5, high magnification: Greater detail of the same embryo showing expression of dmt1 RNA in the intestine of a day 5 embryo.