Delineation of 2ME2 and Dex-induced caspase cascade.

2ME2 induces the activation of caspase-9 (A) and -8 (B). MM.1S cells were treated with 2ME2 (3 μM) and Dex (0.05 μM) and harvested at 48 hours. Cytosolic proteins were separated by 12.5% SDS-PAGE and analyzed by immunoblotting with anti–caspase-8 (cas-8) and -9 (Cas-9) antibodies. Blots are representative of 3 independent experiments. (C) Cleavage of caspase-3 induced by 2ME2. MM.1S cells were treated with 2ME2 (3 μM) and Dex (0.05 μM) and were harvested at 48 hours. Total cell lysates were analyzed by immunoblotting with anti–caspase-3 antibody. FL indicates full length; CF, cleaved fragment. (D) 2ME2 and Dex-induced differential signaling cascades in MM.