Fig. 2.
SU11248 inhibits phosphorylation of FLT3-Asp835.
CHO cells were transiently transfected with DNA encoding wild-type (top) or mutant (bottom) FLT3 and incubated with SU11248 at indicated concentrations for one hour. Following treatment, CHO cells transfected with FLT3-WT were stimulated with FL (100 ng/mL) for 10 minutes. Lysates were prepared and immunoprecipitated with an anti-FLT3 antibody. Following transfer to nitrocellulose, blots were initially probed with an antiphosphotyrosine antibody and subsequently stripped and reprobed with an anti-FLT3 antibody.
