Surface phenotypes of DCs differentiated from ES cells and bone marrow cells.

ES-DCs on day 17 (A), day 26 (B), and when given maturation stimuli (C) were analyzed using flow cytometry on the surface expression of indicated molecules. (C) For analysis on expression of CD11c, CD40, and CD205, cells were stimulated with IL-4, TNF-α, plus LPS, and for the analysis on expression of other molecules, cells stimulated with IL-4, TNF-α, plus anti-CD40 mAb were used. For comparison, DCs generated from bone marrow cells by 10-day culture in the presence of GM-CSF (D) and those stimulated with TNF-α for 20 hours (E) were also analyzed. Staining patterns with specific antibodies (thick lines) and isotype-matched controls (thin dotted lines) are shown.