Fig. 5.
Allogeneic MLR-stimulating capacity of ES-DCs.
(A) Graded numbers of ES-DCs harvested on day 17 (⋄), ES-derived cells on day 10 (■), and (CBA × C57BL/6) F1 BM-DCs cultured for 11 days (○) were irradiated and cocultured with splenic T cells (1.5 × 105) isolated from Balb/c mice for 4 days, and the proliferative response of T cells in the last 16 hours of the culture was measured by [3H]-thymidine uptake. (B) ES-DCs harvested on day 24 and stimulated with IL-4/TNF-α/anti-CD40 (⋄), IL-4/TNF-α/LPS (▵), or IL-4/TNF-α/anti-CD40/LPS (▪) were used as stimulators. BM-DCs cultured for 10 days and stimulated with TNF-α (○) for 20 hours were also used as stimulators. For control, ES-derived cells cultured on OP9 feeder cell layers for 10 days were also used as stimulators (■). Mean values in kilo cpm (kcpm) ± standard deviation for triplicate cultures are shown.