Pretreatment with CD18 mAb reduces apoptosis following brief ischemia but not prolonged ischemia and reperfusion.

The mouse hind limb was subjected to varying periods of ischemia by aortic cross-clamping followed by 3 hours of reperfusion. The percentage of TUNEL-positive nuclei relative to total nuclei in each field was determined following pretreatment with CD18 mAb or hamster IgG. Values represent means ± SEM. Solid bars show results for mice pretreated with CD18 mAb (2 μg/g body weight, administered intravenously, at 16 hours prior to cross-clamping) and open bars show results for controls treated with hamster IgG (2 μg/g body weight). Values represent means ± SEM. *P < .05 vs IgG-pretreated.