Fig. 3.
Arg216Gln GATA-1 dissociates from a palindromic GATA site more easily than wild-type GATA-1.
GATA-1::DNA complexes were formed by incubating either wild-type or Arg216Gln GATA-1 with 32P-labeled single GATA site or palindromic GATA site probes (top). One hundred–fold molar excess of corresponding unlabeled probe was then added att = 0 minute, and reaction mixtures were loaded onto gels at the indicated times (middle panels). The first lane of each set shows migration of the free probe alone. (bottom panels) PhosphorImager quantitation of percentage of bound probe over time (here, in seconds).
