Changes in gene expression following activation of wild-type or Arg216Gln G1E-ER2 by β-estradiol.

Two clones of wild-type or Arg216Gln G1E-ER2 cells were induced with β-estradiol, and transcripts of erythroid genes were assayed by Northern blotting. Like wild-type G1E-ER2 clones, Arg216Gln G1E-ER2 clones showed changes in erythroid gene expression. However, in some instances (eg, the erythroid anion transporter band 3; see “Results” for details), the level of induction was reduced relative to wild-type controls. Parental G1E cells, which lack GATA-1, do not display changes in expression of these markers following β-estradiol induction.