IUHSCT results in stable long-term low-level chimerism and induces donor specific tolerance.
(A) Representative dot plot of peripheral blood discriminating donor and host cells using antibodies specific for H-2Kb and H-2Kd. (B) Levels of chimerism remain stable for more than 6 months. (C) MLR was performed using responder splenocytes from chimeric mice, created by IUHSCT and Balb/c and B6 controls. Splenocyte responders were mixed with host (Balb/c), donor (B6), and third-party (CBA) stimulator cells and the stimulation index (SI) was calculated. Baseline counts per minute (CPM) in control cultures without stimulator cells. SIs were calculated by dividing mean CPM from responses against host (self) (Balb/c), donor (B6), or third party (CBA/J) by mean baseline CPM. *P < .001, #P < .05 when compared to B6 or Balb/c control.
