Typical TCR BV-BC CDR3 size pattern from congenitally infected newborn 2.

cDNA made from total RNA extracted from CBMCs was amplified in 24 PCR reactions, each primed by a BV- and the common BC-specific oligonucleotide. The amplification products were copied in 24 run-off reactions primed by a nested fluorescent BC primer, and the labeled DNA copies were analyzed on a sequencing gel by an automated DNA sequencer. The fluorescent profiles of the 24 BV subfamilies are expressed as fluorescence intensity on the y-axis (arbitrary unit) and BV-BC size on the x-axis.