Expression of γ-globin in MEL cell clones.

MEL cells were transduced at a limiting multiplicity of infection with the vectors depicted in Figure 1, and independent clones were selected with G418. The amount of human γ-globin protein expression was subsequently determined by immunofluorescence staining and flow cytometry following globin gene induction. The presence of intact (●) or recombined vector provirus (○) was determined by Southern blot analysis. Data for vectors HS40-5 and HS40-6 were reported previously.14 15