GVHD in B6 → BALB.B mice.

Splenocytes (2 × 10⁷) along with 5 × 10⁶BM cells from B6 female mice were injected intravenously into irradiated (900 cGy) allogeneic BALB.B male (B6 → BALB.B) or syngeneic (B6 → B6) recipients. (A) Percent survival and mean weight plot (number of survivors indicated for some data points). (B) Recipient leukocytes disappear and transferred allogeneic donor leukocytes appear in the spleen and liver before they are detected in the blood. B6 recipient cells were distinguished from donor cells using the β₂-m^b allotypic marker. In 1B, squares indicate spleen cells; diamonds, liver cells; and circles, PBLs. Open symbols represent recipients and closed symbols represent donors. (C) Seven days after allogeneic transfer, all CD8 cells and a majority of CD4 T cells in host blood are of donor origin (β₂-m^b+). (D) Kinetics of donor-derived CD4 and CD8 T cells from host blood after allogeneic and syngeneic transfer. Data are representative of 2 to 3 independent experiments.