Kinetics of donor-derived resident CD4 and CD8 T cells from lymphoid and nonlymphoid target organs of perfused GVHD mice.

(A) Analysis of spleens and livers 4 days after allogeneic transfer of CFSE-labeled B6 female donor splenocytes plus unlabeled BM into BALB.B male recipients. (B) CD8/CD4 ratios of lymphocytes from spleens, livers, and lungs of hosts after allogeneic (B6 → BALB.B) and syngeneic (BALB.B → BALB.B) transfer. (C) Resident lung leukocytes from B6 → BALB.B mice on day 7 and day 28 after transplantation were analyzed by forward scatter (FSC) and side scatter (SSC) after staining with anti–Mac-1 and –Gr-1 antibodies. Most of the large granular cells on day 28 were Mac-1⁺ and Gr-1⁺. (D) Percentages of Mac-1⁺/Gr-1⁺ lung, liver and splenic leukocytes were determined at time points after allogeneic transfer. In panels A to D, data are from pooled samples from 3 mice and are representative of 3 independent experiments.