H60 dominates the CD8 T-cell response regardless of timing and tissue site.

(A) Serially obtained PBLs from male BALB.B recipients of allogeneic female B6 cells were stained with anti-CD8 mAb and tetramers for H60, H13, and HY minor H Ags and analyzed by flow cytometry. Data are from pooled samples from 10 mice and are representative of multiple experiments. (B) Lymphocytes from the spleens, livers, and lungs from groups of 3 perfused male BALB.B recipients of female B6 cells were prepared at each time point after transfer, stained with the indicated reagents, and analyzed by flow cytometric analysis. Data are representative of 2 independent experiments.