Cell-mediated lympholysis (CML) analysis of cells from GVHD mice.

(A) Analysis of spleens harvested from BALB.B recipients of B6 allogeneic cells on days 7 and 10 after transfer. Splenocytes were cultured in mixed leukocyte culture (MLC) with irradiated splenocytes from male BALB.B stimulator cells. The effector cells were tested for their ability to lyse H60, H28, H4, H13, and HY minor H Ag-peptide loaded, ⁵¹Cr-labeled T2-K^b or T2-D^b target cells. Minor H Ag–specific CTL lines, SP/H60, B6.2/H28, SP/H13, M9, and CTL-10 CTL lines were included as positive controls. (B) Analysis of spleens, livers, and lungs harvested from perfused allogeneic BALB.B recipients on day 10 after transplantation. Data are from pooled organs from 3 mice. Minor H Ag–specific CTL lines were included as positive controls.