Effect of TSA or NaB on osteoclast differentiation in rat bone marrow culture.

Whole rat bone marrow cells or stroma-free rat bone marrow cells for forming MNCs (■) or POCs (░) were cultured as described in “Materials and methods” in the presence of various concentrations of TSA (A) or NaB (B). TSA or NaB was added after 24 hours of incubation. The cells were stained for TRAP, and the number of TRAP⁺ MNCs and POCs was counted in each well. Each bar represents the mean ± SEM of quadruplicate cultures. Data were analyzed by Student t test. *P < .001 compared with the culture without TSA or NaB.