TSA selectively reduces osteoclast-specific mRNA expression.

(A) Stroma-free rat bone marrow cells were cultured for 4 days with or without 15 nM TSA for the formation of POCs. Total RNA was prepared and reverse transcribed, and cDNA was amplified for the number of PCR cycles, using specific primers designed for genes of CTR, cathepsin K, RANK, and GAPDH. The PCR products were stained with ethidium bromide. (B) Relative expression of CTR, cathepsin K, and RANK is shown. The intensity of the bands was determined by densitometry and normalized by the level of GAPDH.