Effect of TSA on fusion of POCs in the presence of sRANKL.

Stroma-free rat bone marrow cells were cultured in 96-well culture plates under the condition to form POCs for 4 days. The cells were then washed 3 times with α-MEM containing 15% FCS and further incubated for 2 days in the presence of 30 ng/mL sRANKL with or without TSA. Cells were then fixed and stained for TRAP. The number of TRAP⁺ MNCs was counted in each well. Each value represents the mean ± SEM of quadruplicate cultures. Data were analyzed by Student t test. *P < .001 compared with the culture without TSA.