TSA does not affect the expression of macrophage-associated phenotypes in mouse bone marrow culture.

(A) RT-PCR analysis of mouse bone marrow macrophage (mBMMs) formed in the presence or absence of TSA. (B) FACS analysis of surface markers Mac-1 and F4/80 of mBMMs formed in the presence or absence of TSA. Mouse whole bone marrow cells were cultured in the presence of hM-CSF (50 ng/mL) with or without TSA (20 nM) for 3 days. Total RNA was prepared and analyzed by RT-PCR (A). Adherent cells were harvested and stained with anti-F4/80 or FITC-conjugated anti–Mac-1 antibody (B).