TSA inhibits the expression of CTR and cathepsin K but not CD11b or F4/80 in RAW-D cells stimulated with sRANKL.

(A) Effect of TSA on the expression of various mRNAs of RAW-D cells stimulated with sRANKL. RAW-D cells were cultured with sRANKL in the presence or absence of TSA (10 nM), and total RNA was prepared. Semiquantitative RT-PCR was used to assess the changes in the expression of various genes. cDNA was amplified for the number of PCR cycles indicated and visualized on agarose gels with ethidium bromide. GAPDH levels were used for comparison. (B) FACS analysis of RAW-D cells. RAW-D cells were treated with sRANKL for 3 days in the presence or absence of TSA (10 nM). TSA was added after 24 hours of incubation. Cells were then stained with antimouse Mac-1 or F4/80 antibodies and analyzed by FACS.