TSA and NaB decrease NF-κB–dependent transactivation in RAW-D cells.

RAW-D cells were transiently transfected with 1 μg NF-κB–dependent reporter plasmid p55IgKLuci or control vector p55Luci. Transiently transfected cells were stimulated with TNF-α or sRANKL for 24 hours. Various concentrations of TSA or NaB were added with TNF-α (20 ng/mL) or sRANKL (100 ng/mL). Renilla luciferase expression vector, pΔTK-RL (0.25 μg), was used as internal control for transfection. Bars represent the mean ± SEM of 3 independent transfections. Data were analyzed by Student t test. *P < .001 compared with the culture without TSA or NaB treatment.