Fig. 6.
Association of ZAP-70 with IgM and CD79b in CLL B cells after BCR ligation.
Lysates were made before and 10 minutes after BCR ligation with anti-μ F(ab′)2 and prepared for immune precipitation with antibodies specific for human IgM (A) or CD79b (B). Samples were loaded onto separate lanes of a polyacrylamide gel for immunoblot analysis with anti–ZAP-70, as indicated by the labeled arrow marked “ZAP-70.” Stripped blots were reprobed for anti-μ (A) or anti-CD79b (B), as indicated by the arrows marked “μ” or “CD79b,” respectively. Each pair of lanes represents the lysates prepared from cells before (−) or after (+) BCR ligation, as indicated at the top of each sample lane. The numbers of the CLL samples are provided above each pair of lanes.
