Expression of MIP-1α and MIP-1β mRNA by MM cells.

Two micrograms of total RNA extracted from various myeloma cell lines was reverse transcribed, and 2 μL of the 20 μL reaction was used for PCR analysis of MIP-1α and MIP-1β, and GAPDH mRNA expression as described in “Materials and methods.” Cell lines used in these experiments were U266 (lane 1), RPMI8226 (lane 2), ARH77 (lane 3), IM-9 (lane 4), TSPC-1 (lane 5), and OPC (lane 6).