Inhibitory effects of OPG on osteoclastogenesis induced by MIP-1α, MIP-1β, and MM cells.

Rabbit bone marrow cells were cultured with rh soluble RANK ligand (sRANKL) at 50 ng/mL, rhMIP-1α at 10 ng/mL, and rhMIP-1β at 10 ng/mL, and cocultured with ARH77 cells in the presence (filled bars) or absence (open bars) of OPG at 1 mg/mL. The data represent the number of TRAP⁺ MNCs and are expressed as a mean of 5 samples with an error bar of SD. * indicates significantly different from the cultures without OPG (P < .05, ANOVA with Scheffe post hoc tests).