Cotreatment with bryostatin 1 dramatically overcomes protection of Bcl-2 against paclitaxel-induced mitochondrial dysfunction.

U937/neo, U937/Bcl-2, and U937/ΔBcl-2 cells were treated with paclitaxel (10 nM) with or without bryostatin 1 (10 nM) for 24 hours. Cytosolic expression of cytochrome c and Smac/DIABLO was monitored by Western analysis as described under “Materials and methods.” Blots were reprobed for actin to ensure equal loading and transfer of protein (data not shown). Two additional studies yielded equivalent results.