Cotreatment of bryostatin 1 and paclitaxel induces equivalent caspase-8 activation and Bid cleavage in parental and cells ectopically expressing Bcl-2 and ΔBcl-2.

U937/neo, U937/Bcl-2, and U937/ΔBcl-2 cells were treated with paclitaxel (10 nM) with or without bryostatin 1 (10 nM) for 24 hours. Cleavage of procaspase-8 and Bid was detected by Western analysis as described in “Materials and methods.” Blots were reprobed for actin to ensure equal loading and transfer of protein. Two additional experiments yielded identical results.