Bryostatin 1 increases paclitaxel-induced apoptosis in cells that have undergone mitotic arrest.

U937/neo (░) and U937/ΔBcl-2 (▪) cells were treated with paclitaxel (10 nM) and bryostatin 1 (10 nM) for 18 hours, after which cells were labeled with the MPM-2 antibody (M) and analyzed by flow cytometry to determine the percentage of cells in mitosis as described under “Materials and methods.” Values represent the means for 3 separate experiments (± SE). *Significant difference compared with treatment with paclitaxel alone in corresponding cells (P < .02).